ZnT4 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86336-1-RR (same clone as 86336-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86336-1-RR (ZnT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86336-1-PBS in a different storage buffer formulation.

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  Sample test of MP02364-1

  The mean ZnT4 concentration was determined to be 45.6 ng/mL in LNCaP cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human prostate cancer using 86336-1-RR (same clone as 86336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86336-1-RR (ZnT4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86336-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02364-1

  Cytometric bead array standard curve of MP02364-1, ZnT4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86336-2-PBS. Detection antibody: 86336-1-PBS. Standard: Ag3313. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86336-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86336-1-RR against Human ZnT4 were performed. The affinity constant is 3.87 nM.

ZnT4 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86336-1-PBS

Clone No.250909D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4

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Product Information

86336-1-PBS targets ZnT4 as part of a matched antibody pair:

MP02364-1: 86336-2-PBS capture and 86336-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZnT4 fusion protein Ag3313
• Full Name: solute carrier family 30 (zinc transporter), member 4
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC026089
• Gene Symbol: ZNT4
• Gene ID (NCBI): 7782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14863
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZnT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZnT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZnT4 is expressed in numerous tissues and is enriched in the prostate. ZnT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).