ZnT4 Recombinant antibody

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  WB analysis using 86336-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86336-1-RR (ZnT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 86336-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86336-1-RR (ZnT4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86336-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86336-1-RR against Human ZnT4 were performed. The affinity constant is 3.87 nM.

ZnT4 Recombinant Antibody for WB, IHC, ELISA

Cat No. 86336-1-RR

Clone No.250909D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4

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Tested Applications

• Positive WB detected in: LNCaP cells, mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86336-1-RR targets ZnT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZnT4 fusion protein Ag3313
• Full Name: solute carrier family 30 (zinc transporter), member 4
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC026089
• Gene Symbol: ZNT4
• Gene ID (NCBI): 7782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14863
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZnT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZnT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZnT4 is expressed in numerous tissues and is enriched in the prostate. ZnT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).