SLC35A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC35A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16342-1-AP

Synonyms

CMP-Sia-Tr, CMP-sialic acid transporter, CMP-SA-Tr, CMP sialic acid transporter, CMP Sia Tr

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Validation Data Gallery

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC35A1 antibody (16342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse spleen tissue, mouse testis tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16342-1-AP targets SLC35A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC35A1 fusion protein Ag9524
• Full Name: solute carrier family 35 (CMP-sialic acid transporter), member A1
• Calculated Molecular Weight: 337 aa, 37 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC017807
• Gene Symbol: SLC35A1
• Gene ID (NCBI): 10559
• RRID: AB_2190077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Solute carrier family 35 member A1 (SLC35A1) , also named as CMP-sialic acid transporter (CST) , is responsible for transferring CMP-sialic acid from the cytosol through Golgi membranes, thus playing a vital role in the terminal sialylation of glycan structures. Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. In addition, SCL35A1 acts independently from sialic acid in functional α-DG O-mannosylation(PMID: 25552652).

Publications

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	WB, IF	Cells; Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays.; Authors - Antje Banning ; KO Validated
human	WB	Cell Rep; A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.; Authors - Jessie Kulsuptrakul ; KO Validated