Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13657-1-AP targets SLC35A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4524 Product name: Recombinant human SLC35A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC035747 Sequence: MAAVGAGGSTAAPGPGAVSAGALEPGTASAAHRRLKYISLAVLVVQNASLILSIRYARTLPGDRFFATTAVVMAEVLKGLTCLLLLFAQKRGNVKHLVLFLHEAVLVQYVDTLKLAVPSLIYTLQNNLQYVAISNLPAATFQPSPRCSQSHSLCLCLRLRALRSPAASRAATTTAAVFPPWRPHHGALSAKVSAGEVRAGSNGGTQGRGTGVEGVGHLQDPSRHPPGPGSSGFGRWSFLPGH Predict reactive species |
| Full Name | solute carrier family 35 (UDP-galactose transporter), member A2 |
| Calculated Molecular Weight | 396 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa, 32 kDa |
| GenBank Accession Number | BC035747 |
| Gene Symbol | SLC35A2 |
| Gene ID (NCBI) | 7355 |
| RRID | AB_2877967 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78381 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC35A2 antibody 13657-1-AP | Download protocol |
| WB protocol for SLC35A2 antibody 13657-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC35A2 Polyclonal antibody (Cat# 13657-1-AP) has a total of 5 citations. These publications appear in Frontiers in Immunology, Cell Oncology, FEBS Journal, Gene, and Aging. The associated research fields span hepatocellular carcinoma metastasis, breast cancer progression via ERK pathway activation, osteosarcoma proliferation and invasion through mitophagy regulation, colorectal cancer prognosis, and comprehensive multi-cancer immunotherapeutic analysis.
| Species | Application | Title |
|---|---|---|
Front Immunol Comprehensive analysis of 33 human cancers reveals clinical implications and immunotherapeutic value of the solute carrier family 35 member A2 | ||
Cell Oncol (Dordr) Nucleotide sugar transporter SLC35A2 is involved in promoting hepatocellular carcinoma metastasis by regulating cellular glycosylation
| ||
Gene Solute carrier family 35 member A2 regulates mitophagy through the PI3K/AKT/mTOR axis, promoting the proliferation, migration, and invasion of osteosarcoma cells | ||
Aging (Albany NY) Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer |











