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Tested Applications
| Positive WB detected in | L02 cells, MCF-7 cells, MDA-MB-231 cells, U2OS cells, MG-63 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31436-1-AP targets SLC35B3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34349 Product name: Recombinant human SLC35B3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-77 aa of BC006973 Sequence: MDLTQQAKDIQNITVQETNKNNSESIECSKITMDLKFNNSRKYISITVPSKTQTMSPHIKSVDDVVVLGMNLSKFNK Predict reactive species |
| Full Name | solute carrier family 35, member B3 |
| Calculated Molecular Weight | 401 aa, 45 kDa |
| Observed Molecular Weight | 40, 32 kDa |
| GenBank Accession Number | BC006973 |
| Gene Symbol | SLC35B3 |
| Gene ID (NCBI) | 51000 |
| RRID | AB_3742427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H1N7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC35B3 (solute carrier family 35 member B3) is a member of the solute carrier family. SLC35B3 is involved in transporting 3-prime phosphoadenosine 5-prime phosphosulfate (PAPS) from the nucleus or the cytosol to the Golgi lumen. The observed MW of SLC35B3 is 40 kDa and 32 kDa, which is due to alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC35B3 antibody 31436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
