SLC36A4 Polyclonal antibody

SLC36A4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24066-1-AP

Synonyms

PAT4, SLC36A4

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Validation Data Gallery

• 

  WB analysis of A549 using 24066-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24066-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24066-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24066-1-AP is SLC36A4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC36A4 fusion protein Ag21142
• Full Name: solute carrier family 36 (proton/amino acid symporter), member 4
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 41-66 kDa
• GenBank Accession Number: BC047374
• Gene Symbol: SLC36A4
• Gene ID (NCBI): 120103
• RRID: AB_2879424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC36A4 (also known as proton-coupled amino-acid transporter 4, PAT4), is a member of the solute carrier (SLC) 36 family which functions as transporter for amino acids and derivatives. SLC36A4 is widely distributed at the mRNA level in normal tissues and many cancer cell lines. It modulates the mTORC1 signaling and is essential for the proliferation of cancer cells. In addition, small GTPase Rab12 controls the degradation of SLC36A4, which indirectly modulates mTORC1 activity and autophagy. This antibody can recognize the endogenous SLC36A4 protein in multiple cell lines.