SLC38A4 Polyclonal antibody

SLC38A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 20857-1-AP

Synonyms

Amino acid transporter A3, ATA3, NAT3, PAAT, SLC38A4, SNAT4

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Validation Data Gallery

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  WB analysis using 20857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20857-1-AP (SLC38A4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, L02 cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

20857-1-AP targets SLC38A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC38A4 fusion protein Ag14827
• Full Name: solute carrier family 38, member 4
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC101827
• Gene Symbol: SLC38A4
• Gene ID (NCBI): 55089
• RRID: AB_10696186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB,IF	Pathol Res Pract; Assisted reproductive technology causes reduced expression of amino acid transporters in human full-term placentas; Authors - Qingge Jia
pig	WB,IF	J Clin Invest; Arteriovenous metabolomics in pigs reveals CFTR regulation ofmetabolism inmultiple organs; Authors - Hosung Bae

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christopher (Verified Customer) (06-13-2024)

EXCELLENT ANTIBODY! I had very clean bands using a 1:1000 dilution with the recommended blocking buffers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Placenta tissue