Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16343-1-AP targets SLC3A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9525 Product name: Recombinant human SLC3A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-685 aa of BC022386 Sequence: IPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHANELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKIRIRLSTNSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILYTSC Predict reactive species |
| Full Name | solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 |
| Calculated Molecular Weight | 685 aa, 79 kDa |
| Observed Molecular Weight | 78-90 kDa |
| GenBank Accession Number | BC022386 |
| Gene Symbol | SLC3A1 |
| Gene ID (NCBI) | 6519 |
| RRID | AB_2239419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q07837 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC3A1 antibody 16343-1-AP | Download protocol |
| IP protocol for SLC3A1 antibody 16343-1-AP | Download protocol |
| WB protocol for SLC3A1 antibody 16343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC3A1 Polyclonal antibody (Cat# 16343-1-AP) has 12 citations spanning journals including Cell Stem Cell, Nature Cell Biology, Nature Communications, Theranostics, Genes & Diseases, Developmental Cell, Molecular Therapy Nucleic Acids, Scientific Reports, FASEB Journal, BMC Nephrology, PLoS One, and Animal Science Journal. Research fields cover renal development and differentiation, cystinuria, stem cell biology, breast cancer, metabolic disorders, and nutrient transport in the gut.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation | ||
Nat Cell Biol Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. | ||
Nat Commun Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney. | ||
Theranostics Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.
| ||
Dev Cell AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis. |
Reviews
What customers say
The antibody performed well for immunohistochemistry, successfully localizing derived human stem cells in sectioned, paraffin-embedded mouse tissue at a 1:200 dilution. It was also noted as suitable for immunofluorescence. Overall, the reviewer rated it 5 stars, describing it as a reliable reagent for IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Felisha (Verified Customer) (01-03-2020) | The primary antibody worked great to localize the derived human stem cells in the mouse tissue samples that were sectioned and paraffin embedded. Wonderful for immunohistochemistry!
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