SLC44A1 Polyclonal antibody

SLC44A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14687-1-AP

Synonyms

CD92, CDW92, choline transporter-like protein 1, CHTL1, CTL1, SLC44A1

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Validation Data Gallery

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  WB analysis using 14687-1-AP

  HeLa lysates (heated at 100℃ and 37℃) were subjected to SDS PAGE followed by western blot with 14687-1-AP (SLC44A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14687-1-AP (SLC44A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14687-1-AP (SLC44A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: 37°C incubated HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

14687-1-AP targets SLC44A1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC44A1 fusion protein Ag6024
• Full Name: solute carrier family 44, member 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: BC049203
• Gene Symbol: SLC44A1
• Gene ID (NCBI): 23446
• RRID: AB_10640573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC44A1, also referred to as choline transporter-like protein 1 (CTL1) or CDw92, is a choline transporter detected in both the plasma and mitochondrial membranes where it transports choline at high affinity and in a Na+-independent manner.

Publications

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	IHC	Cell Prolif; The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation; Authors - Nanxin Liu
human	IHC	BMC Cancer; Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy; Authors - Bingjie Guan
mouse	IF	Cell Metab; Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.; Authors - Elsa Sanchez-Lopez