Tested Applications
| Positive WB detected in | 37°C incubated HeLa cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14687-1-AP targets SLC44A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6024 Product name: Recombinant human SLC44A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 55-208 aa of BC049203 Sequence: AARLVSGYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGV Predict reactive species |
| Full Name | solute carrier family 44, member 1 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 70-73 kDa |
| GenBank Accession Number | BC049203 |
| Gene Symbol | SLC44A1 |
| Gene ID (NCBI) | 23446 |
| RRID | AB_10640573 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWI5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC44A1, also referred to as choline transporter-like protein 1 (CTL1) or CDw92, is a choline transporter detected in both the plasma and mitochondrial membranes where it transports choline at high affinity and in a Na+-independent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC44A1 antibody 14687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC44A1 Polyclonal antibody (Cat# 14687-1-AP) has 8 citations published in Cell Metabolism, Nature Communications, Cell Death & Disease, Journal of Experimental Medicine, Cell Proliferation, Biochemical Pharmacology, and BMC Cancer. Associated research fields include choline metabolism and transport, macrophage inflammation, mitochondrial biogenesis, retinal homeostasis, acute myeloid leukemia chemotherapy resistance, intestinal nutrient absorption, melanoma drug resistance, and rectal cancer prognosis.
| Species | Application | Title |
|---|---|---|
Cell Metab Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity | ||
Cell Death Dis The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia. | ||
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Cell Prolif The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation |
