SLC4A4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC4A4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 11885-1-AP

Synonyms

hhNMC, HNBC1, KNBC, kNBC1, Na(+)/HCO3( ) cotransporter, NBC, NBC1, NBC2, NBCE1, pNBC, SLC4A4, SLC4A5

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Validation Data Gallery

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  WB analysis of HEK-293 using 11885-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11885-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11885-1-AP

  IP result of anti-SLC4A4 (IP:11885-1-AP, 4ug; Detection:11885-1-AP 1:400) with HEK-293 cells lysate 1200ug.

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  IHC staining of human kidney using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11885-1-AP

  Immunofluorescent analysis of HepG2 cells, using SLC4A4 antibody 11885-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Product Information

11885-1-AP targets SLC4A4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC4A4 fusion protein Ag2490
• Full Name: solute carrier family 4, sodium bicarbonate cotransporter, member 4
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC030977
• Gene Symbol: SLC4A4
• Gene ID (NCBI): 8671
• RRID: AB_2191458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
mouse	WB	Int J Mol Sci; Modulation of Tubular pH by Acetazolamide in a Ca2+ Transport Deficient Mice Facilitates Calcium Nephrolithiasis.; Authors - Eugenia Awuah Boadi
mouse	IHC	Am J Physiol Gastrointest Liver Physiol; Expression of the regulated isoform of the electrogenic Na+/HCO3- cotransporter, NBCe1, is enriched in pacemaker interstitial cells of Cajal.; Authors - Maria-Gabriela Colmenares Aguilar ; KO Validated
human	IHC	Am J Physiol Renal Physiol; Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney.; Authors - Hyun-Wook Lee
mouse	WB	Front Physiol; Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel.; Authors - Bruce Chen
	WB,IHC	Front Physiol; Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.; Authors - Shengjie Luo