SLC5A11 Polyclonal antibody

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  WB analysis using 28648-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28648-1-AP (SLC5A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 28648-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 28648-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 28648-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC5A11 antibody (28648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28648-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC5A11 antibody (28648-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC5A11 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 28648-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28648-1-AP targets SLC5A11 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29916

  Product name: Recombinant human SLC5A11 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 166-229 aa of BC057780

  Sequence: EGLKEKYFLALASNRSENSSCGLPREDAFHIFRDPLTSDLPWPGVLFGMSIPSLWYWCTDQVIV

• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 11
• Calculated Molecular Weight: 611 aa, 67 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC057780
• Gene Symbol: SLC5A11
• Gene ID (NCBI): 115584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWX8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC5A11 (Sodium/myo-inositol cotransporter 2 ), also known as KST1, SMIT2, is a sodium/solute cotransporter-like neuronal protein. In Drosophila, SLC5A11 mediates hunger by regulating potassium channel activity. SLC5A11 has been linked to infantile convulsions and paroxysmal dyskinesia (ICCA syndrome) and benign familial infantile convulsions (BFIC) (PMID: 27397890).