Various lysates were subjected to SDS PAGE followed by western blot with 28648-1-AP (SLC5A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 28648-1-AP (SLC5A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 28648-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 28648-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 28648-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC5A11 antibody (28648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC5A11 antibody (28648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 28648-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC5A11 antibody (28648-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC5A11 antibody (28648-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse small intestine tissue
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
28648-1-AP targets SLC5A11 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SLC5A11 (Sodium/myo-inositol cotransporter 2 ), also known as KST1, SMIT2, is a sodium/solute cotransporter-like neuronal protein. In Drosophila, SLC5A11 mediates hunger by regulating potassium channel activity. SLC5A11 has been linked to infantile convulsions and paroxysmal dyskinesia (ICCA syndrome) and benign familial infantile convulsions (BFIC) (PMID: 27397890).
Various lysates were subjected to SDS PAGE followed by western blot with 28648-1-AP (SLC5A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 28648-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 28648-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse small intestine using 28648-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC5A11 antibody (28648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 28648-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC5A11 antibody (28648-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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