SLC6A9 / GlyT1 Polyclonal antibody

• 

  WB analysis using 30971-1-AP

  BxPC-3 cells lysates were subjected to SDS PAGE followed by western blot with 30971-1-AP (SLC6A9 / GlyT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

SLC6A9 / GlyT1 Polyclonal Antibody for WB, ELISA

Cat No. 30971-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

---

Tested Applications

• Positive WB detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30971-1-AP targets SLC6A9 / GlyT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC6A9 / GlyT1 fusion protein Ag34437
• Full Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 9
• Calculated Molecular Weight: 706 aa, 78 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_201649
• Gene Symbol: SLC6A9
• Gene ID (NCBI): 6536
• RRID: AB_3669799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC6A9, also named glycine transporter 1 (GlyT1), is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. GlyT1 is the main regulator of synaptic glycine concentrations, assisted by the neuronal GlyT2 (PMID: 28828604). The 55-60 kDa band is the partially glycosylated form of GlyT1 (PMID: 27874011, PMID: 8995423).