Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31867-1-AP targets SLX4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36095 Product name: Recombinant human BTBD12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1530-1834 aa of BC036335 Sequence: MPSAGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN Predict reactive species |
| Full Name | BTB (POZ) domain containing 12 |
| Calculated Molecular Weight | 1834 aa, 200 kDa |
| Observed Molecular Weight | 180-200 kDa |
| GenBank Accession Number | BC036335 |
| Gene Symbol | BTBD12 |
| Gene ID (NCBI) | 84464 |
| RRID | AB_3742487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8IY92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLX4 (Structure-specific endonuclease subunit SLX4) is a scaffold protein that assembles multiple structure-specific nucleases, including SLX1, MUS81-EME1, and XPF-ERCC1. These nucleases are essential for resolving complex DNA structures that arise during DNA replication, repair, and recombination (PMID: 34804132). SLX4 is a key protein involved in DNA repair and genome stability, with significant implications for genetic disorders and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLX4 antibody 31867-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alexandros (Verified Customer) (02-06-2026) | excellent performance on western on PVDF membranes and 5% milk as blocking agent. easy to distinguish the correct band based on the molecular weight
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