SMAD6 Polyclonal antibody

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  WB analysis using 18342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18342-1-AP (SMAD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SMAD6 Polyclonal Antibody for WB, ELISA

Cat No. 18342-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hSMAD6, MADH6, Mothers against DPP homolog 6, SMAD family member 6

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Tested Applications

• Positive WB detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18342-1-AP targets SMAD6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD6 fusion protein Ag13128
• Full Name: SMAD family member 6
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012986
• Gene Symbol: SMAD6
• Gene ID (NCBI): 4091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43541
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD6 is an intracellular signal transduction protein that belongs to the SMAD protein family. It acts as a negative regulator in the transforming growth factor-beta (TGF-β) signaling pathway, binding to TGF-β receptors and inhibiting the phosphorylation of SMAD2/SMAD3, thereby blocking the transmission of TGF-β signals. Additionally, SMAD6 is involved in the regulation of the bone morphogenetic protein (BMP) signaling pathway, affecting cell proliferation, differentiation, and apoptosis. During development, SMAD6 is crucial for the formation of bones and cartilage. Its abnormal expression is associated with various diseases, including skeletal developmental abnormalities and certain types of cancer.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.; Authors - Wei Lu