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SMAP2 Recombinant monoclonal antibody (88105-1-RR)

SMAP2 Recombinant monoclonal antibody

SMAP2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88105-1-RR

Clone No. 260364G2

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 88105-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88105-1-RR (SMAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 88105-1-RR
Biolayer interferometry (BLI) kinetic assays of 88105-1-RR against Human SMAP2 were performed. The affinity constant is 2.41 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

LNCaP cells, PC-3 cells, K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88105-1-RR targets SMAP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag9751 Product name: Recombinant human SMAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-429 aa of BC021133 Sequence: QIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDKWKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKDLDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK Predict reactive species
Full Name	small ArfGAP2
Calculated Molecular Weight	429 aa, 47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC021133
Gene Symbol	SMAP2
Gene ID (NCBI)	64744
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8WU79
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAP2 (Small Arf GAP Protein 2) is an Arf1 GTPase-activating protein. It localizes to early endosomes/TGN, binds clathrin/AP-1, and regulates retrograde transport from recycling endosomes to the Golgi. Critical for vesicle trafficking, organelle homeostasis, and spermatogenesis. (PMID: 26136365)