SMARCA2-Specific Recombinant antibody

• 

  WB analysis using 85018-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 85018-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 85018-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85018-4-RR

Clone No.242511E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

SMARCA2, BRM, BRG1-associated factor 190B, BAF190B, BAF190

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85018-4-RR targets SMARCA2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMARCA2-Specific fusion protein Ag25157
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_003070
• Gene Symbol: SMARCA2
• Gene ID (NCBI): 6595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51531
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.