SMC4 Polyclonal antibody, PBS Only

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• SMC4 Polyclonal antibody, PBS Only (24758-1-PBS)

• 

  WB analysis using 24758-1-AP (same clone as 24758-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24758-1-AP (same clone as 24758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24758-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

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Product Information

24758-1-PBS targets SMC4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19156

  Product name: Recombinant human SMC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 992-1288 aa of BC106033

  Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII

• Full Name: structural maintenance of chromosomes 4
• Calculated Molecular Weight: 1288 aa, 147 kDa
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: BC106033
• Gene Symbol: SMC4
• Gene ID (NCBI): 10051
• RRID: AB_2879707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NTJ3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.