SMC4 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86182-1-RR (same clone as 86182-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86182-1-RR (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86182-1-RR (same clone as 86182-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC4 antibody (86182-1-RR, Clone: 250782E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

SMC4 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86182-1-PBS

Clone No.250782E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

---

Product Information

86182-1-PBS targets SMC4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19156

  Product name: Recombinant human SMC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 992-1288 aa of BC106033

  Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII

• Full Name: structural maintenance of chromosomes 4
• Calculated Molecular Weight: 1288 aa, 147 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC106033
• Gene Symbol: SMC4
• Gene ID (NCBI): 10051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NTJ3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.