Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, C6 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86182-1-RR targets SMC4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SMC4 fusion protein Ag19156 Predict reactive species |
Full Name | structural maintenance of chromosomes 4 |
Calculated Molecular Weight | 1288 aa, 147 kDa |
Observed Molecular Weight | 170-180 kDa |
GenBank Accession Number | BC106033 |
Gene Symbol | SMC4 |
Gene ID (NCBI) | 10051 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NTJ3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.
Protocols
Product Specific Protocols | |
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WB protocol for SMC4 antibody 86182-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |