SMC4 Recombinant antibody

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  WB analysis using 86182-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86182-1-RR (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SMC4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86182-1-RR

Clone No.250782E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86182-1-RR targets SMC4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMC4 fusion protein Ag19156
• Full Name: structural maintenance of chromosomes 4
• Calculated Molecular Weight: 1288 aa, 147 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC106033
• Gene Symbol: SMC4
• Gene ID (NCBI): 10051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NTJ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.