Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25589-1-AP targets SMCHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22293 Product name: Recombinant human SMCHD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1813-2005 aa of BC035774 Sequence: LLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDV Predict reactive species |
| Full Name | structural maintenance of chromosomes flexible hinge domain containing 1 |
| Calculated Molecular Weight | 2005 aa, 226 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC035774 |
| Gene Symbol | SMCHD1 |
| Gene ID (NCBI) | 23347 |
| RRID | AB_2880144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NHR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) is considered a non-canonical member of the SMC family owing to differences in its type of ATPase domain and in its overall linear domain architecture (PMID: 32779700). SMCHD1 is best known for its role in promoting DNA methylation and gene silencing, with apparent roles in X chromosome inactivation, imprinted gene regulation, and autosome gene cluster repression (PMID: 31668908). Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia syndrome (BAMS)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| IHC protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| IP protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| WB protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMCHD1 Polyclonal antibody (Cat# 25589-1-AP) has 3 citations published in Genome Biology, Frontiers in Cell and Developmental Biology, and PLoS Pathogens. The research fields span epigenetics and meiotic DNA repair, metabolic-immune crosstalk in myocardial infarction, and post-translational modification (SUMOylation/ubiquitination) regulation of antiviral defense against herpes simplex virus 1.
| Species | Application | Title |
|---|---|---|
Genome Biol The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots | ||
Front Cell Dev Biol Metabolic-immune crosstalk in myocardial infarction: RLF and SMCHD1 identified as causal therapeutic targets via integrated lactylation-MR analysis. | ||
PLoS Pathog SUMOylation and ubiquitination reciprocally regulate SMCHD1 antiviral activity against herpes simplex virus 1. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user reported excellent performance detecting SMCHD1 in HeLa cells using a 1:1000 dilution with overnight incubation at 4°C, describing the antibody as working "very fine." Overall, the feedback is positive, confirming reliable detection under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (10-05-2025) | Works very fine to detect SMCHD1 in HeLa cells- 1/1000 incubation, ON at 4°C.
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