SMCHD1 Polyclonal antibody

SMCHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25589-1-AP

Synonyms

KIAA0650, SMCHD1

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Validation Data Gallery

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  WB analysis of HeLa using 25589-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25589-1-AP (SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25589-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25589-1-AP (SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25589-1-AP

  IP result of anti-SMCHD1 (IP:25589-1-AP, 4ug; Detection:25589-1-AP 1:1000) with HeLa cells lysate 1085 ug.

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  IHC staining of mouse skeletal muscle using 25589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 25589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 25589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

25589-1-AP targets SMCHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMCHD1 fusion protein Ag22293
• Full Name: structural maintenance of chromosomes flexible hinge domain containing 1
• Calculated Molecular Weight: 2005 aa, 226 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC035774
• Gene Symbol: SMCHD1
• Gene ID (NCBI): 23347
• RRID: AB_2880144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) is considered a non-canonical member of the SMC family owing to differences in its type of ATPase domain and in its overall linear domain architecture (PMID: 32779700). SMCHD1 is best known for its role in promoting DNA methylation and gene silencing, with apparent roles in X chromosome inactivation, imprinted gene regulation, and autosome gene cluster repression (PMID: 31668908). Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia syndrome (BAMS)

Publications

Species	Application	Title
human,mouse	WB,IP	Genome Biol; The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots; Authors - Shenli Yuan