SMNDC1 Polyclonal antibody

SMNDC1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12178-1-AP

Synonyms

30 kDa splicing factor SMNrp, SMN related protein, SMNDC1, SMNR, SPF30

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Validation Data Gallery

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  WB analysis using 12178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12178-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12178-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12178-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12178-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12178-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 12178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human lung tissue, human liver tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12178-1-AP targets SMNDC1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMNDC1 fusion protein Ag2822
• Full Name: survival motor neuron domain containing 1
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC011234
• Gene Symbol: SMNDC1
• Gene ID (NCBI): 10285
• RRID: AB_2239665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).