• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SMO Monoclonal antibody, PBS Only

SMO Monoclonal Antibody for IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine

Applications

IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11A10

Cat no : 66851-2-PBS

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using SMO Monoclonal antibody (66851-2-Ig)

    IF Staining of MDCK using 66851-2-Ig (same clone as 66851-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using SMO antibody (66851-2-Ig, Clone: 2A11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 66851-2-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66851-2-PBS targets SMO in IF, Indirect ELISA applications and shows reactivity with Human, Canine samples.

Tested Reactivity Human, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMO fusion protein Ag25861
Full Name smoothened homolog (Drosophila)
Calculated Molecular Weight 787 aa, 86 kDa
GenBank Accession NumberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.