SMO Monoclonal antibody

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  IF Staining of MDCK using 66851-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using SMO antibody (66851-2-Ig, Clone: 2A11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

SMO Monoclonal Antibody for IF/ICC, ELISA

Cat No. 66851-2-Ig

Clone No.2A11A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine

Applications

IF/ICC, ELISA

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66851-2-Ig targets SMO in IF/ICC, ELISA applications and shows reactivity with Human, Canine samples.

• Tested Reactivity: Human, Canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25861

  Product name: Recombinant human SMO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 28-233 aa of BC009989

  Sequence: RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY

• Full Name: smoothened homolog (Drosophila)
• Calculated Molecular Weight: 787 aa, 86 kDa
• GenBank Accession Number: BC009989
• Gene Symbol: SMO
• Gene ID (NCBI): 6608
• RRID: AB_3085033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99835
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.