SMPDL3A Polyclonal antibody

• 

  WB analysis using 16310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16310-1-AP (SMPDL3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SMPDL3A Polyclonal Antibody for WB, ELISA

Cat No. 16310-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

2',3'-cGAMP phosphodiesterase SMPDL3A, Acid sphingomyelinase-like phosphodiesterase 3a, ASM like phosphodiesterase 3a, ASM3A, ASML3A

---

Tested Applications

• Positive WB detected in: NR8383 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16310-1-AP targets SMPDL3A in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9340

  Product name: Recombinant human SMPDL3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 103-453 aa of BC018999

  Sequence: SFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY

• Full Name: sphingomyelin phosphodiesterase, acid-like 3A
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC018999
• Gene Symbol: SMPDL3A
• Gene ID (NCBI): 10924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a secreted metallophosphoesterase belonging to the calcineurin-like phosphoesterase superfamily, closely related to acid sphingomyelinase (ASMase). Despite its name, SMPDL3A lacks activity against sphingomyelin and instead functions as a nucleotide phosphodiesterase, hydrolyzing nucleoside di- and triphosphates such as ATP. The enzyme contains a binuclear zinc center in its active site and exhibits an acidic pH optimum. The expression of SMPDL3A is induced by cholesterol loading and liver X receptor (LXR) ligands. Recent studies have identified SMPDL3A as a negative regulator of innate immunity by degrading 2'3'-cGAMP, thereby inhibiting the cGAS-STING signaling pathway.