SMPDL3A Polyclonal antibody, PBS Only

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• SMPDL3A Polyclonal antibody, PBS Only (16310-1-PBS)

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  WB analysis using 16310-1-AP (same clone as 16310-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16310-1-AP (SMPDL3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16310-1-PBS in a different storage buffer formulation.

SMPDL3A Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16310-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

2',3'-cGAMP phosphodiesterase SMPDL3A, Acid sphingomyelinase-like phosphodiesterase 3a, ASM like phosphodiesterase 3a, ASM3A, ASML3A

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Product Information

16310-1-PBS targets SMPDL3A in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9340

  Product name: Recombinant human SMPDL3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 103-453 aa of BC018999

  Sequence: SFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY

• Full Name: sphingomyelin phosphodiesterase, acid-like 3A
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC018999
• Gene Symbol: SMPDL3A
• Gene ID (NCBI): 10924
• RRID: AB_3741882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92484
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a secreted metallophosphoesterase belonging to the calcineurin-like phosphoesterase superfamily, closely related to acid sphingomyelinase (ASMase). Despite its name, SMPDL3A lacks activity against sphingomyelin and instead functions as a nucleotide phosphodiesterase, hydrolyzing nucleoside di- and triphosphates such as ATP. The enzyme contains a binuclear zinc center in its active site and exhibits an acidic pH optimum. The expression of SMPDL3A is induced by cholesterol loading and liver X receptor (LXR) ligands. Recent studies have identified SMPDL3A as a negative regulator of innate immunity by degrading 2'3'-cGAMP, thereby inhibiting the cGAS-STING signaling pathway.