SMTNL2 Polyclonal antibody

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  WB analysis of COLO 320 using 14782-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 14782-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 14782-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14782-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 14782-1-AP

  IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

• 

  IF Staining of MCF-7 using 14782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMTNL2 antibody (14782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SMTNL2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14782-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2

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Tested Applications

• Positive WB detected in: COLO 320 cells, MCF-7 cells, mouse small intestine tissue, SGC-7901 cells
• Positive IP detected in: COLO 320 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14782-1-AP targets SMTNL2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6395

  Product name: Recombinant human SMTNL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-317 aa of BC112177

  Sequence: MRKTSNSCIMENGHQPGTGPGDGPPEIAQNFSAPDPPRPRPVSLSLRLPHQPVTAITRVSDRFSGETSAAALSPMSAATLGGLNPSPSEVITPWTPSPSEKNSSFTWSVPSSGYGAVTASKHSNSPPLVTPPQSPVSPQPPAITQVHRQGERRRELVRSQTLPRTSEAQARKALFEKWEQETAAGKGKGEARARLKRSQSFGVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNSLSPTQRQKNFELAFTMAENLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLRRFE

• Full Name: smoothelin-like 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC112177
• Gene Symbol: SMTNL2
• Gene ID (NCBI): 342527
• RRID: AB_2270842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2TAL5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.