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  • KD/KO Validated

SMURF1 Polyclonal antibody

SMURF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55175-1-AP

Synonyms

hSMURF1, KIAA1625, SMURF1



Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55175-1-AP targets SMURF1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SMAD specific E3 ubiquitin protein ligase 1
Calculated molecular weight 86 kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_020429
Gene symbol SMURF1
Gene ID (NCBI) 57154
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

Protocols

Product Specific Protocols
WB protocol for SMURF1 antibody 55175-1-APDownload protocol
IHC protocol for SMURF1 antibody 55175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Cell Biol

A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.

Authors - Ling Guo
humanIHC,IP,WB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Immunol

MLN4924, a First-in-Class NEDD8-Activating Enzyme Inhibitor, Attenuates IFN-β Production.

Authors - Hui Song
  • KD Validated
mouseWB

Biomed Pharmacother

Fucoxanthin rescues dexamethasone induced C2C12 myotubes atrophy.

Authors - Liao Zhiyin
mouseWB

Ecotoxicol Environ Saf

Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.

Authors - Di Wang
humanIHC

Ann Transl Med

SMURF1-mediated ubiquitylation of SHP-1 promotes cell proliferation and invasion of endometrial stromal cells in endometriosis.

Authors - Yunmeng Bian
  • KD Validated