CoraLite® Plus 488-conjugated SMYD3 Monoclonal antibody

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  WB analysis using CL488-66330

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66330 (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD3 Monoclonal Antibody for WB

Cat No. CL488-66330

Clone No.1B5C10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

bA74P14.1, FLJ21080, SMYD3, ZMYND1, ZNFN3A1

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66330 targets SMYD3 in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2624

  Product name: Recombinant human SMYD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 60-428 aa of BC031010

  Sequence: MRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDRKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS

• Full Name: SET and MYND domain containing 3
• Calculated Molecular Weight: 428aa,49 kDa; 369aa,42 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC031010
• Gene Symbol: SMYD3
• Gene ID (NCBI): 64754
• RRID: AB_3084234
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H7B4
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) .