SNF8 Monoclonal antibody

• 

  WB analysis using 67696-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67696-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67696-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67696-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67696-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67696-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67696-1-Ig

Clone No.2C10F2

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2C10F2, Dot3, EAP30, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, U2OS cells, BT-474 cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67696-1-Ig targets SNF8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30384

  Product name: Recombinant human SNF8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-258 aa of BC008976

  Sequence: MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP

• Full Name: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC008976
• Gene Symbol: SNF8
• Gene ID (NCBI): 11267
• RRID: AB_2882888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96H20
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy; Authors - Takashi Nozawa
mouse	WB	J Ethnopharmacol; The stems of Syringa oblata Lindl. exert cardioprotective effects against acute myocardial ischemia by inhibiting the TLR4/MyD88/NF-κB and NLRP3 inflammasome signaling pathways in mice; Authors - Jixuan Xu