SNF8 Monoclonal antibody, PBS Only

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  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67696-1-Ig (same clone as 67696-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67696-1-PBS

Clone No.2C10F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2C10F2, Dot3, EAP30, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22

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Product Information

67696-1-PBS targets SNF8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30384

  Product name: Recombinant human SNF8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-258 aa of BC008976

  Sequence: MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP

• Full Name: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC008976
• Gene Symbol: SNF8
• Gene ID (NCBI): 11267
• RRID: AB_2882888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96H20
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.