SNRPA Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 86259-3-RR (same clone as 86259-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA antibody (86259-3-RR, Clone: 251055G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86259-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86259-3-RR against Human SNRPA were performed. The affinity constant is below 1 pM.

SNRPA Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86259-3-PBS

Clone No.251055G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

U1 A, U1 snRNP A, U1A, U1-A

---

Product Information

86259-3-PBS targets SNRPA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0283

  Product name: Recombinant human SNRPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-258 aa of BC000405

  Sequence: KKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAG

• Full Name: small nuclear ribonucleoprotein polypeptide A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC000405
• Gene Symbol: SNRPA
• Gene ID (NCBI): 6626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09012
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.