SNRPA Recombinant monoclonal antibody

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  WB analysis using 86259-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86259-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86259-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA antibody (86259-3-RR, Clone: 251055G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86259-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86259-3-RR against Human SNRPA were performed. The affinity constant is below 1 pM.

SNRPA Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86259-3-RR

Clone No.251055G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

U1 A, U1 snRNP A, U1A, U1-A

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse brain tissue, HepG2 cells, HeLa cells, A431 cells, HEK-293 cells, rat thymus tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86259-3-RR targets SNRPA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0283

  Product name: Recombinant human SNRPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-258 aa of BC000405

  Sequence: KKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAG

• Full Name: small nuclear ribonucleoprotein polypeptide A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC000405
• Gene Symbol: SNRPA
• Gene ID (NCBI): 6626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09012
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.