SNRPA1 Polyclonal antibody

SNRPA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17368-1-AP

Synonyms

U2 snRNP A', U2 snRNP A, U2 small nuclear ribonucleoprotein A'

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Validation Data Gallery

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  WB analysis of HeLa using 17368-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17368-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17368-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17368-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17368-1-AP

  IP result of anti-SNRPA1 (IP:17368-1-AP, 4ug; Detection:17368-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17368-1-AP (SNRPA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17368-1-AP (SNRPA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17368-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA1 antibody (17368-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, Raji cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung tissue, human brain tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human spleen tissue, human stomach cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

17368-1-AP targets SNRPA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPA1 fusion protein Ag10790
• Full Name: small nuclear ribonucleoprotein polypeptide A'
• Calculated Molecular Weight: 255 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC022816
• Gene Symbol: SNRPA1
• Gene ID (NCBI): 6627
• RRID: AB_2193724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.; Authors - Yajie Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Cell Rep; SF3B1 Association with Chromatin Determines Splicing Outcomes.; Authors - Nir Kfir
human	WB	STAR Protoc; Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.; Authors - Li Wang
human	WB,IHC	Clin Respir J; High SNRPA1 expression leads to poor prognosis in patients with lung adenocarcinoma; Authors - Juan Juan Yang