SNRPB Recombinant antibody

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  WB analysis using 86099-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86099-1-RR (SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86099-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of K-562 using 86099-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNRPB Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86099-1-RR

Clone No.250577G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, Jurkat cells, Ramos cells, K-562 cells, NIH/3T3 cells
• Positive IF/ICC detected in: Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86099-1-RR targets SNRPB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNRPB fusion protein Ag10298
• Full Name: small nuclear ribonucleoprotein polypeptides B and B1
• Calculated Molecular Weight: 234aa,25 kDa; 285aa,30 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC003530
• Gene Symbol: SNRPB
• Gene ID (NCBI): 6628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14678
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.