SNUPN Polyclonal antibody

SNUPN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15358-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Snurportin-1, Snurportin1, snurportin 1, RNUT1, RNA U transporter 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 15358-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15358-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 15358-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 15358-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 15358-1-AP
Immunofluorescent analysis of MCF-7 cells, using SNUPN antibody 15358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 15358-1-AP
Immunofluorescent analysis of HeLa cells using 15358-1-AP (SNUPN antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa cells using 15358-1-AP
IF result of 15358-1-AP from Dr. Ralph Kehlenbach.

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15358-1-AP targets SNUPN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7604 Product name: Recombinant human SNUPN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC004203 Sequence: MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN Predict reactive species
Full Name	snurportin 1
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC004203
Gene Symbol	SNUPN
Gene ID (NCBI)	10073
RRID	AB_2192073
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95149
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SNUPN antibody 15358-1-AP	Download protocol
IHC protocol for SNUPN antibody 15358-1-AP	Download protocol
WB protocol for SNUPN antibody 15358-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SNUPN Polyclonal antibody (Cat# 15358-1-AP) has 2 total citations. One appears in Nature Communications (2024), studying SNUPN deficiency causing recessive muscular dystrophy through RNA mis-splicing and ECM dysregulation. The other is in Neurobiology of Disease (2020), investigating UsnRNP trafficking regulated by stress granules and compromised by mutant ALS proteins. Both studies used human samples with WB and/or IF applications.

Species	Application	Title
human	WB,IF	Nat Commun SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation Authors - Marwan Nashabat View Article
human	WB	Neurobiol Dis UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins. Authors - Simona Rossi View Article