CoraLite® Plus 488-conjugated SNX17 Monoclonal antibody

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  IF Staining of HeLa using CL488-68256

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SNX17 antibody (CL488-68256, Clone: 3B8F6 ) at dilution of 1:200, CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using CL488-68256

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SNX17 antibody (CL488-68256, Clone: 3B8F6 ) at dilution of 1:200.

SNX17 Monoclonal Antibody for IF/ICC

Cat No. CL488-68256

Clone No.3B8F6

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

IF/ICC

Synonyms

KIAA0064, SNX17, sorting nexin 17

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68256 targets SNX17 in IF/ICC applications and shows reactivity with Human, rat, pig samples.

• Tested Reactivity: Human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32624

  Product name: Recombinant human SNX17 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 17-226 aa of BC002524

  Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA

• Full Name: sorting nexin 17
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002524
• Gene Symbol: SNX17
• Gene ID (NCBI): 9784
• RRID: AB_3084460
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15036
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.