CoraLite® Plus 488-conjugated SNX18 Monoclonal antibody

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  IF Staining of A431 using CL488-68305

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 SNX18 antibody (CL488-68305, Clone: 2E7D6 ) at dilution of 1:200.

SNX18 Monoclonal Antibody for IF/ICC

Cat No. CL488-68305

Clone No.2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF/ICC

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68305 targets SNX18 in IF/ICC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17388

  Product name: Recombinant human SNX18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 55-284 aa of BC060791

  Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

• Full Name: sorting nexin 18
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC060791
• Gene Symbol: SNX18
• Gene ID (NCBI): 112574
• RRID: AB_3084470
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96RF0
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).