SNX3 Monoclonal antibody

SNX3 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B5B7

Cat no : 68292-1-Ig

Synonyms

Grd19, MCOPS8, Protein SDP3, SDP3, SNX3, sorting nexin 3

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Validation Data Gallery

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  WB analysis using 68292-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68292-1-Ig (SNX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 68292-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (68292-1-Ig, Clone: 2B5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68292-1-Ig targets SNX3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNX3 fusion protein Ag1160
• Full Name: sorting nexin 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC008444
• Gene Symbol: SNX3
• Gene ID (NCBI): 8724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).