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SNX6 Polyclonal antibody

SNX6 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 10114-1-AP

Synonyms

MSTP010, SNX6, sorting nexin 6, TFAF2, TRAF4 associated factor 2



Tested Applications

Positive WB detected inRAW 264.7 cells, HepG2 cells, human placenta tissue, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman heart tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10114-1-AP targets SNX6 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX6 fusion protein Ag0168
Full Name sorting nexin 6
Calculated molecular weight 34 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001798
Gene symbol SNX6
Gene ID (NCBI) 58533
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants

Protocols

Product Specific Protocols
WB protocol for SNX6 antibody 10114-1-APDownload protocol
IHC protocol for SNX6 antibody 10114-1-APDownload protocol
IP protocol for SNX6 antibody 10114-1-APDownload protocol
FC protocol for SNX6 antibody 10114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Authors - Pengfei Hu
  • KD Validated