SOAT2 Polyclonal antibody

SOAT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21852-1-AP

Synonyms

EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2

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Validation Data Gallery

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  WB analysis using 21852-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21852-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IHC detected in: human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

21852-1-AP targets SOAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOAT2 fusion protein Ag16364
• Full Name: sterol O-acyltransferase 2
• Calculated Molecular Weight: 522 aa, 60 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC096090
• Gene Symbol: SOAT2
• Gene ID (NCBI): 8435
• RRID: AB_11183043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491)

Publications

Species	Application	Title
human	IHC	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu