SOD2 Recombinant monoclonal antibody

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  WB analysis using 83519-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83519-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83519-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 83519-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HL-60 using 83519-4-RR

  1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  FC experiment of A549 using 83519-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SOD2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83519-4-RR

Clone No.240462H8

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

SODM, 240462H8, EC:1.15.1.1, IPO B, IPOB

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells
• Positive FC (Intra) detected in: A549 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83519-4-RR targets SOD2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21388

  Product name: Recombinant human SOD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-140 aa of BC016934

  Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV

• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• RRID: AB_3671145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P04179
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dhanwini (Verified Customer) (09-24-2025)

GOOD

• Applications: Western Blot

FH

Robert (Verified Customer) (09-24-2025)

Used for western and CE-SDS. Expect bands to look similar to the images provided by proteintech.

• Applications: Western Blot, Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HCEC, HCET

FH

Sneha (Verified Customer) (09-24-2025)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

FH

Michael (Verified Customer) (09-23-2025)

Excellent results from this!

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Corneal cells

FH

Henry (Verified Customer) (09-23-2025)

hELPED FOR SPECIFIC RESULTS

• Applications: Western Blot, Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:4500
• Cell Tissue Type: MIOM1 PBMCS