CoraLite® Plus 488-conjugated SOD2 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-83519-2

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SOD2 antibody (CL488-83519-2, Clone: 240462F8 ) at dilution of 1:200.

• 

  IF Staining of HeLa using CL488-83519-2

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SOD2 antibody (CL488-83519-2, Clone: 240462F8 ) at dilution of 1:200.

SOD2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-83519-2

Clone No.240462F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83519-2 targets SOD2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21388

  Product name: Recombinant human SOD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-140 aa of BC016934

  Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV

• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• RRID: AB_3673264
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04179
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.