SOHLH1 Polyclonal antibody

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  IHC staining of human testis using 17753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17753-1-AP (SOHLH1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17753-1-AP (SOHLH1 Antibody) at dilution of 1:100 (under 40x lens).

SOHLH1 Polyclonal Antibody for IHC, ELISA

Cat No. 17753-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

C9orf157, NOHLH, SOHLH1, TEB2

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17753-1-AP targets SOHLH1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12107

  Product name: Recombinant human SOHLH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC031861

  Sequence: MASRCSEPYPEVSRIPTVRGCNGSLSGALSCCEDSAQGSGPPKAPTVAEGPSSCLRRNVISERERRKRMSLSCERLRALLPQFDGRREDMASVLEMSVQFLRLASALGPSQEQHAILASSKEMWHSLQEDVLQLTLSSQIQAGVPDPGTGASSGTRTPDVKAFLESPWSLDPASASPEPVPHILASSRQWDPASCTSLGTDKCEALLGLCQVRGGLPPFSEPSSLVPWPPGRSLPKAVRPPLSWPPFSQQQTLPVMSGEALGWLGQAGSLAMGAAPLGEPAKEDPMLAQEAGSALGSDVDDGTSFLLTAGPSSWPGEWGPGFRAGPPA

• Full Name: spermatogenesis and oogenesis specific basic helix-loop-helix 1
• Calculated Molecular Weight: 328 aa, 34 kDa
• GenBank Accession Number: BC031861
• Gene Symbol: SOHLH1
• Gene ID (NCBI): 402381
• RRID: AB_2878434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5JUK2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spermatogenesis‐and oogenesis‐specific helix‐loop‐helix transcription factor 1 (SOHLH1) is a gonad‐specific transcription factor that belongs to the basic‐helix‐loop‐helix (bHLH) transcription factor family. SOHLH1 has a profound effect on the development of germ cells, from SSC self-maintenance to spermatogonium differentiation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB, IF	Hum Mol Genet; The mutation c.346-1G > a in SOHLH1 impairs sperm production in the homozygous but not in the heterozygous condition.; Authors - Mohan Liu