Tested Applications
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17753-1-AP targets SOHLH1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12107 Product name: Recombinant human SOHLH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC031861 Sequence: MASRCSEPYPEVSRIPTVRGCNGSLSGALSCCEDSAQGSGPPKAPTVAEGPSSCLRRNVISERERRKRMSLSCERLRALLPQFDGRREDMASVLEMSVQFLRLASALGPSQEQHAILASSKEMWHSLQEDVLQLTLSSQIQAGVPDPGTGASSGTRTPDVKAFLESPWSLDPASASPEPVPHILASSRQWDPASCTSLGTDKCEALLGLCQVRGGLPPFSEPSSLVPWPPGRSLPKAVRPPLSWPPFSQQQTLPVMSGEALGWLGQAGSLAMGAAPLGEPAKEDPMLAQEAGSALGSDVDDGTSFLLTAGPSSWPGEWGPGFRAGPPA Predict reactive species |
| Full Name | spermatogenesis and oogenesis specific basic helix-loop-helix 1 |
| Calculated Molecular Weight | 328 aa, 34 kDa |
| GenBank Accession Number | BC031861 |
| Gene Symbol | SOHLH1 |
| Gene ID (NCBI) | 402381 |
| RRID | AB_2878434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JUK2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spermatogenesis‐and oogenesis‐specific helix‐loop‐helix transcription factor 1 (SOHLH1) is a gonad‐specific transcription factor that belongs to the basic‐helix‐loop‐helix (bHLH) transcription factor family. SOHLH1 has a profound effect on the development of germ cells, from SSC self-maintenance to spermatogonium differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SOHLH1 antibody 17753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SOHLH1 affinity-purified antibody (Cat# 17753-1-AP) has 2 total citations. These appear in Human Molecular Genetics and Nan Fang Yi Ke Da Xue Xue Bao. The research fields span reproductive biology (SOHLH1 mutation effects on sperm production) and oncology (SOHLH1-mediated ferroptosis in osteosarcoma). Applications used include Western blotting and immunofluorescence in human samples.
| Species | Application | Title |
|---|---|---|
Hum Mol Genet The mutation c.346-1G > a in SOHLH1 impairs sperm production in the homozygous but not in the heterozygous condition. | ||
Nan Fang Yi Ke Da Xue Xue Bao [Polyphyllin VII inhibits osteosarcoma xenograft growth in mice by inducing ferroptosis via upregulating SOHLH1]. |
