Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, human testis tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83787-5-RR targets SON in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag27620 Product name: Recombinant human SON protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-182 aa of NM_001291411 Sequence: MVSKFREIQQELSSGRNEGQLNGETNTPIEGNQAGDAAASARSLPNEEIVQKIEEVLSGVLDTELRYKPDLKEGSRKSRCVSVQTDPTDEIPTKKSKKHKKHKNKKKKKKKEKEKKYKRQPEESESKTKSHDDGNIDLESDSFLKFDSEPSAVALELPTRAFGPSETNES Predict reactive species |
| Full Name | SON DNA binding protein |
| Calculated Molecular Weight | 264 aa |
| Observed Molecular Weight | 250-300 kDa |
| GenBank Accession Number | NM_001291411 |
| Gene Symbol | SON |
| Gene ID (NCBI) | 6651 |
| RRID | AB_3671379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18583 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SON antibody 83787-5-RR | Download protocol |
| IHC protocol for SON antibody 83787-5-RR | Download protocol |
| WB protocol for SON antibody 83787-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SON Recombinant monoclonal antibody (240845B7), catalog number 83787-5-RR, has 4 citations. These appear in Advanced Science, Cell Reports, International Journal of Molecular Sciences, and Nature Cell Biology. Associated research fields include cervical cancer growth and serine metabolism reprogramming, nuclear genome organization and folding, osteoporosis-osteoarthritis comorbidity, and nuclear speckle biology with intron-retained RNA regulation.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) HNRNPU K181 Lactylation Drives Cervical Cancer Growth by Upregulating PHGDH and Reprogramming Serine Metabolism. | ||
Int J Mol Sci Targeting Osteoporosis-Osteoarthritis Comorbidity: Multi-Omics Identification of SON and Exploration of Therapeutic Agents. | ||
Nat Cell Biol The periphery of nuclear speckles defines a spatially and temporally regulated compartment of long-lived intron-retained RNAs that resolves during mitosis. |
