SOS1 Polyclonal antibody

SOS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55041-1-AP

Synonyms

GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1

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Validation Data Gallery

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  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55041-1-AP

  IP result of anti-SOS1 (IP:55041-1-AP, 4ug; Detection:55041-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOS1 antibody (55041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55041-1-AP targets SOS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: son of sevenless homolog 1 (Drosophila)
• Calculated Molecular Weight: 152 kDa
• Observed Molecular Weight: 152 kDa
• GenBank Accession Number: NM_005633
• Gene Symbol: SOS1
• Gene ID (NCBI): 6654
• RRID: AB_10804659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.

Publications

Species	Application	Title
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	Pathol Res Pract; KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway; Authors - Jishuang Ding
human	WB	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang