SOX5 Recombinant monoclonal antibody, PBS Only

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• SOX5 Recombinant monoclonal antibody, PBS Only (86619-2-PBS)

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  WB analysis using 86619-2-RR (same clone as 86619-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 86619-2-RR (same clone as 86619-2-PBS)

  WB result of SOX5 antibody (86619-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX5 transfected U-87 MG cells. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 86619-2-RR (same clone as 86619-2-PBS)

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86619-2-RR (same clone as 86619-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 86619-2-RR (same clone as 86619-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

SOX5 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86619-2-PBS

Clone No.251512D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

L SOX5, Transcription factor SOX-5

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Product Information

86619-2-PBS targets SOX5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4092

  Product name: Recombinant human SOX5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-376 aa of BC029220

  Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN

• Full Name: SRY (sex determining region Y)-box 5
• Calculated Molecular Weight: 763 aa, 84 kDa
• Observed Molecular Weight: 84-90 kDa
• GenBank Accession Number: BC029220
• Gene Symbol: SOX5
• Gene ID (NCBI): 6660
• RRID: AB_3745013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35711
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SOX5 is a member of the SRY-related HMG-box (SOX) family of transcription factors, which play critical roles in cell fate determination and differentiation during embryonic development. SOX5 contains a highly conserved high-mobility group (HMG) DNA-binding domain that enables it to regulate target gene expression by bending DNA and facilitating chromatin remodeling. SOX5 is essential for chondrogenesis, neurogenesis, and gliogenesis, and its dysregulation has been implicated in various cancers and developmental disorders (PMID: 19647094, PMID: 30914279). SOX5 has two known protein isoforms: a short isoform of 48 kDa and a long isoform of 84 kDa (PMID: 21732189). This antibody detects the 84-90 kDa band and has passed KD/KO validation.