Filter: AllWBIF/ICC

WB analysis using 86619-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of U-87 MG using 86619-2-RR
WB result of SOX5 antibody (86619-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX5 transfected U-87 MG cells.
WB analysis of mouse brain using 86619-2-RR
Mouse brain tissue were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 86619-2-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of A549 using 86619-2-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KD/KO Validated

SOX5 Recombinant monoclonal antibody

Name: SOX5 Recombinant monoclonal antibody
Brand: Proteintech
SKU: 86619-2-RR

SOX5 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86619-2-RR

Clone No.251512D11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

L SOX5, Transcription factor SOX-5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, mouse brain tissue, U-87 MG cells, NCI-H1299 cells
Positive IF/ICC detected in	HepG2 cells, A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86619-2-RR targets SOX5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4092 Product name: Recombinant human SOX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-376 aa of BC029220 Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN Predict reactive species
Full Name	SRY (sex determining region Y)-box 5
Calculated Molecular Weight	763 aa, 84 kDa
Observed Molecular Weight	84-90 kDa
GenBank Accession Number	BC029220
Gene Symbol	SOX5
Gene ID (NCBI)	6660
RRID	AB_3745013
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35711
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX5 is a member of the SRY-related HMG-box (SOX) family of transcription factors, which play critical roles in cell fate determination and differentiation during embryonic development. SOX5 contains a highly conserved high-mobility group (HMG) DNA-binding domain that enables it to regulate target gene expression by bending DNA and facilitating chromatin remodeling. SOX5 is essential for chondrogenesis, neurogenesis, and gliogenesis, and its dysregulation has been implicated in various cancers and developmental disorders (PMID: 19647094, PMID: 30914279). SOX5 has two known protein isoforms: a short isoform of 48 kDa and a long isoform of 84 kDa (PMID: 21732189). This antibody detects the 84-90 kDa band and has passed KD/KO validation.