SP3 Recombinant antibody, PBS Only

• 

  WB analysis using 85050-1-RR (same clone as 85050-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85050-1-RR (SP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85050-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 85050-1-RR (same clone as 85050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SP3 antibody (85050-1-RR, Clone: 242556A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85050-1-PBS in a different storage buffer formulation.

SP3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85050-1-PBS

Clone No.242556A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242556A2, SPR 2, SPR-2

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Product Information

85050-1-PBS targets SP3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SP3 fusion protein Ag25082
• Full Name: Sp3 transcription factor
• Calculated Molecular Weight: 781 aa, 82 kDa
• Observed Molecular Weight: 70 kDa, 115 kDa
• GenBank Accession Number: BC126414
• Gene Symbol: SP3
• Gene ID (NCBI): 6670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02447
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SP3, also named as transcription factor Sp3, is a 781 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. SP3 localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. SP3 is acetylated by histone acetyltransferase p300, deacetylated by HDACs and sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. There exits tow modified isoform and molecular weight of those are 70 kda and 115 kda.