SPAM1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86527-1-RR (same clone as 86527-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86527-1-RR (SPAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86527-1-PBS in a different storage buffer formulation.

SPAM1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86527-1-PBS

Clone No.251297F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:3.2.1.35, HYA1, Hyal PH20, HYAL1, HYAL3

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Product Information

86527-1-PBS targets SPAM1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1682

  Product name: Recombinant human SPAM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-511 aa of BC026163

  Sequence: LLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSA

• Full Name: SPAM1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC026163
• Gene Symbol: SPAM1
• Gene ID (NCBI): 6677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P38567
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.