SPAM1 Recombinant monoclonal antibody

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• SPAM1 Recombinant monoclonal antibody (86527-1-RR)

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  WB analysis using 86527-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86527-1-RR (SPAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SPAM1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86527-1-RR

Clone No.251297F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:3.2.1.35, HYA1, Hyal PH20, HYAL1, HYAL3

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Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells, SH-SY5Y cells, A-673 cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86527-1-RR targets SPAM1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1682

  Product name: Recombinant human SPAM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-511 aa of BC026163

  Sequence: LLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSA

• Full Name: SPAM1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC026163
• Gene Symbol: SPAM1
• Gene ID (NCBI): 6677
• RRID: AB_3744944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P38567
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.